Multiple Decay Analysis of the Correlation FunctionĤ.1. Crossover Exponential, Phase Separation, and Chain DimensionalityĬhapter 4. Flexible Branched Molecules and Starsģ.15. Excluded Volume Effect on Translational Diffusion of Linear Moleculesģ.14. Diffusion of Linear Polymers under Theta Conditionsģ.12. Diffusion of Random-Flight, Linear Moleculesģ.10. Anisotropic Translational Diffusion of Cylindersģ.9. Determination of the Equivalent Hydrodynamic Shape of Irregular Rigid Structuresģ.8. Determination of the Equivalent Hydrodynamic Shape of Regular Solidsģ.7. Determination of the Molecular Weightģ.6. Friction Factors Associated with DTr and Dmģ.5. Effect of External Field-Sedimentationģ.4. Macroscopic Description of Mass Transportģ.3. Translational Diffusion-Hydrodynamic Dissipationģ.1. Dynamic Light Scattering by Absorbing MoleculesĬhapter 3. Electric Field and Intensity Correlation FunctionsĢ.16. Light Scattering by Large, Interacting Particles: One-Contact ApproximationĢ.12. Light Scattering by Small, Interacting ParticlesĢ.9. Light Scattering by Large, Noninteracting ParticlesĢ.8. Light Scattering by Small, Noninteracting ParticlesĢ.7. Total (Integrated) Intensity of Scattered LightĢ.6. Brief History of Dynamic Light ScatteringĬhapter 2. This textbook is intended for (1) advanced undergraduate students and graduate students in the chemical, physical, and biological sciences (2) scientists who might wish to apply DLS methods to systems of interest to them but who have no formal training in the field of DLS and (3) those who are simply curious as to the type of information that might be obtained from DLS techniques. Since a major emphasis of this textbook is the interpretation of DLS data obtained by polarized light scattering studies on macromolecular solutions, the results of complementary experimental techniques are also presented in order to gain insight into the dynamics of these systems. Problems are presented at the end of each chapter to emphasize these concepts. The material is presented in order of increasing complexity of the systems under examination, ranging from dilute solutions of noninteracting particles to concentrated multicomponent solutions of strongly interacting particles and gels. It presents the appropriate equations used to interpret DLS data. Every point is illustrated by case studies, including a commercially available albumin, a small RNA virus isolated from plants, as well as four soluble proteins and a ribonucleoprotein assembly purified and characterized by students in the frame of their master degree.An Introduction to Dynamic Light Scattering by Macromolecules provides an introduction to the basic concepts of dynamic light scattering (DLS), with an emphasis on the interpretation of DLS data. Variables like temperature, solvent viscosity, and inter-particle interactions may also influence particle size determination. To ensure reproducible quantitative data, attention should be paid to controlling the preparation and handling of proteins or assemblies because variations in the state of aggregation, induced by minor changes in experimental condition or technique, might compromise DLS results and affect protein activity. It reviews the basic concepts of light scattering measurements and addresses four critical aspects of the analysis and interpretation of DLS results. This article is written for graduate and undergraduate students with access to DLS and for faculty members who wish to incorporate DLS into a lab activity, a practical course or research. Dynamic light scattering (DLS) analyses are routinely used in biology laboratories to detect aggregates in macromolecular solutions, to determine the size of proteins, nucleic acids, and complexes or to monitor the binding of ligands.
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